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Series 13 of Holby City commenced on 19 October 2010 and finished on 11 October 2011; it comprised 52 episodes.
Main Characters
- Robert Powell as Mark Williams
- Amanda Mealing as Connie Beauchamp
- Guy Henry as Henrik Hanssen
- Hugh Quarshie as Ric Griffin
- Paul Bradley as Elliot Hope
- Hari Dhillon as Michael Spence
- Adam Astill as Dan Hamilton
- Rosie Marcel as Jac Naylor
- Luke Roberts as Joseph Byrne
- Bob Barrett as Sacha Levy
- Edward MacLiam as Greg Douglas
- Jimmy Akingbola as Antoine Malick
- Laila Rouass as Sahira Shah
- James Anderson as Oliver Valentine
- Emma Catherwood as Penny Valentine
- Tina Hobley as Chrissie Williams
- Jaye Jacobs as Donna Jackson
- Patsy Kensit as Faye Byrne
- Olga Fedori as Frieda Petrenko
- Sarah-Jane Potts as Eddie McKee
- La Charné Jolly as Elizabeth Tait
- Lauren Drummond as Chantelle Lane
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| Series 12 | Series 14 |
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