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As part of the BBC's DoNation season, Casualty and Holby City join forces for a special, interactive episode in which viewers vote to determine the outcome of an organ donation storyline.
- Simon MacCorkindale as Harry Harper
- Susan Cookson as Maggie Coldwell
- Luisa Bradshaw-White as Lisa Fox
- James Redmond as John "Abs" Denham
- Suzanne Packer as Tess Bateman
- Sharon D Clarke as Lola Griffin
- Ian Bleasdale as Josh Griffiths
- Martina Laird as Comfort Jones
- Matthew Wait as Luke Warren
- Rebekah Gibbs as Nina Farr
- Annette Badland as Wendy Wincott
- Hywel Bennett as David Wincott
- Steven Pinder as Tony Harvey
- Nina Wadia as Jean
- Sarah Bedi as Lucy Beddows
- Brian Cowan as Alan Beddows
- Yvonne O'Grady as Carol Beddows
- Emily Hamilton as Sarah Wincott
- David Alderman as Matt Wincott
- Matthew Pidgeon as Chris Thomas
- Nila Aalia as Sheila
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